August 27, 2020
Mass screening program using highly sensitive RIA kits
Prostate-specific antigen ranges from a mass screening program utilizing extremely delicate RIA kits.
BACKGROUND
This examine was designed to guage the distribution of prostate-specific antigen (PSA) ranges of males in a mass screening program for prostatic illness.
METHODS
A complete of 763 males over 40 years of age underwent mass screening for prostatic illness in a Japanese prefecture utilizing the extremely delicate Eiken package and the Hybritech (Tandem-R) package. The screening exams consisted of serum PSA willpower, digital rectal examination, a questionnaire on signs, analysis of prostate quantity, and willpower of the weight problems price.
RESULTS
Serum PSA ranges of all topics had been measured with each kits. The correlation between the values obtained by the Eiken package and people of the Tandem-R package was excessive (r = 0.990), however the values of the previous had been barely greater than these of the latter. Serum PSA weakly correlated with age, nevertheless, when estimated inside decade age brackets, the degrees of PSA confirmed important variations amongst only one pair of teams stratified by age. In distinction, the degrees of PSA confirmed important variations amongst 9 pairs of teams stratified by prostate quantity.
CONCLUSIONS
A extremely delicate assay package is beneficial to guage each the distribution of PSA ranges and the connection of PSA values to age and prostate quantity of males in mass screening packages for prostatic illness, since roughly 40% of the topics who underwent the current mass screening confirmed PSA values underneath 1.Zero ng/mL, which have been the the decrease restrict of detection of many PSA kits.
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250G Glycine |
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1KG Glycine Ultrapure |
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250G Tris |
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100G Tricine |
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100G Ion Exchange Resin (mixed bed) |
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| NAT1056 | National Diagnostics | 100G | EUR 96 |
25G Riboflavin |
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25ML TEMED |
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100G Ammonium Persulfate |
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1G Dithiothreitol (DTT) |
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5G Dithiothreitol (DTT) |
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50ML 2-Mercaptoethanol |
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450ML Glycerol Ultrapure |
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1L Tween 20 |
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| NAT1084 | National Diagnostics | 1L | EUR 169.2 |
200ML Formamide |
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100G EDTA |
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Calibration of the Amersham Ferritin RIA package utilizing the WHO human liver ferritin worldwide commonplace 80/602.
A business ferritin package (Amersham Ferritin RIA (A)) was calibrated utilizing the WHO human liver ferritin worldwide commonplace 80/602 (W). The reconstituted WHO freeze-dried commonplace was diluted to acquire 5 focus ranges starting from 10-800 micrograms/l.
Logarithmic transformation of the values was carried out as a way to stabilize the variance, yielding the regression equation: logA = 0.0235 + 1.0022 logW. The slope of the regression line (being very near, and never considerably totally different from, one) was set to 1, and the relation between the untransformed values then turned a proportionality: A = 1.067 x W.
A WHO commonplace ferritin worth of 15 micrograms/l (usually used as cut-off worth for absent iron reserves) and of 30 mu/l (usually used as threshold worth for the presence of stainable marrow haemosiderin iron) yielded calculated Amersham Ferritin RIA values of 16.Zero micrograms/l and 32.Zero micrograms/l.
[Radioimmunoassay for the pyridinoline cross-linked carboxy-terminal telopeptide of type 1 collagen (1CTP)–some basic aspects of the RIA kit and clinical evaluation in various bone diseases].
A radioimmunoassay for circulating ranges of the pyridinoline cross-linked carboxy-terminal telopeptide of kind 1 collagen (1CTP) was developed and will be accessible as a package on a business base. Utilizing the kits, we evaluated principally and clinically the assay.
- The assayed values had been reproducible and the assay can detect as little as 0.5 ng/ml of 1CTP. In wholesome volunteers, circulating degree was excessive underneath age 24 and over age 46. In sufferers with bone metastasis, serum ranges elevated even in its early stage and correlated properly with scientific standing.
- In different bone ailments, corresponding to major hyperparathyroidism, hyperthyroidism, post-gastrectomy, hypercalcemia of malignancy and myeloma, serum ranges elevated in accordance with their scientific circumstances. In sufferers with persistent renal failure, serum ranges had been excessive, suggesting lower of renal clearance of 1CTP. The circulating 1CTP ranges appeared to mirror properly scientific bone damaging standing.
- A excessive correlation between serum 1CTP degree and urinary pyridinoline (r = 0.884) was proven, whereas primarily no correlation was noticed between bone formation markers corresponding to osteocalcin and alkaline phosphatase. Thus, the measurement of circulating 1CTP appears to be a easy and delicate methodology to watch bone destruction.
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25G Bis (Methylene Bis-Acrylamide) |
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25G DATD Ultrapure |
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100G Tricine |
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25G Riboflavin |
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25ML TEMED |
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100G Ammonium Persulfate |
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1G Dithiothreitol (DTT) |
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5G Dithiothreitol (DTT) |
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50ML 2-Mercaptoethanol |
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100G SDS Ultrapure |
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1KG SDS Ultrapure |
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250G Urea |
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1KG Urea |
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450ML Glycerol Ultrapure |
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200ML Tween 20 Ultrapure |
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1L Tween 20 |
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200ML Formamide |
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500ML Boric Acid |
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100G EDTA |
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500G EDTA |
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25ML Formamide CE |
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1L DEPC Treated Water |
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Sterling Rapid Silver Stain Kit |
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| NAT1106 | National Diagnostics | 1KIT | EUR 265.2 |
1L ProtoBlue Safe |
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| NAT1108 | National Diagnostics | 1L | EUR 159.6 |
4L ProtoBlue Safe |
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| NAT1110 | National Diagnostics | 4L | EUR 282 |
1L ProtoStain Blue |
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25ML Nuclistain |
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450ML AcrylaGel 30% |
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1L AcrylaGel 30% |
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450ML UreaGel 29:1 Concentrate |
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UreaGel 29:1 System 1L Kit |
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450ML SequaGel UreaGel Concentrate |
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1L SequaGel UreaGel Concentrate |
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SequaGel Sequencing System 1L Kit |
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100ML SequaGel Buffer |
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200ML SequaGel UreaGel Buffer |
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1L SequaGel UreaGel-6 |
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1L SequaGel UreaGel-8 |
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| NAT1150 | National Diagnostics | 1L | EUR 151.2 |
450ML SequaGel UreaGel Diluent |
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| NAT1152 | National Diagnostics | 450ML | EUR 90 |
1L SequaGel UreaGel Diluent |
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| NAT1154 | National Diagnostics | 1L | EUR 103.2 |
90ML SequaGel Complete Buffer |
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| NAT1156 | National Diagnostics | 90ML | EUR 79.2 |
200ML SequaGel UreaGel Complete Buffer |
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| NAT1158 | National Diagnostics | 200ML | EUR 85.2 |
450ML SequaGel XR |
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| NAT1160 | National Diagnostics | 450ML | EUR 126 |
1L SequaGel XR |
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| NAT1162 | National Diagnostics | 1L | EUR 165.6 |
100ML SequaGel XR Concentrate |
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| NAT1164 | National Diagnostics | 100ML | EUR 128.4 |
450ML SequaGel XR Concentrate |
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| NAT1166 | National Diagnostics | 450ML | EUR 361.2 |
200ML SequaGel MD Monomer Solution |
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| NAT1168 | National Diagnostics | 200ML | EUR 220.8 |
SequaGel MD SSCP Kit |
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| NAT1170 | National Diagnostics | 1KIT | EUR 253.2 |
SequaGel MD Heteroduplex Kit |
|||
| NAT1172 | National Diagnostics | EACH | EUR 265.2 |
1.2ML SequaGel MD SSCP Stop Solution |
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| NAT1174 | National Diagnostics | 1.2ML | EUR 85.2 |
450ML AccuGel 19:1 (30%) |
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| NAT1176 | National Diagnostics | 450ML | EUR 126 |
1L AccuGel 19:1 (30%) |
|||
| NAT1178 | National Diagnostics | 1L | EUR 165.6 |
450ML AccuGel 19:1 (40%) |
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| NAT1180 | National Diagnostics | 450ML | EUR 130.8 |
1L AccuGel 19:1 (40%) |
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| NAT1182 | National Diagnostics | 1L | EUR 168 |
450ML AccuGel 29:1 (30%) |
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| NAT1184 | National Diagnostics | 450ML | EUR 126 |
1L AccuGel 29:1 (30%) |
|||
| NAT1186 | National Diagnostics | 1L | EUR 165.6 |
450ML AccuGel 29:1 (40%) |
|||
| NAT1188 | National Diagnostics | 450ML | EUR 130.8 |
1L AccuGel 29:1 (40%) |
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| NAT1190 | National Diagnostics | 1L | EUR 168 |
1.2ML Triple Dye Loading Buffer (6X) |
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| NAT1192 | National Diagnostics | 1.2ML | EUR 85.2 |
UreaGel Loading Buffer |
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| NAT1194 | National Diagnostics | 10X1ML | EUR 222 |
1L TBE 10X |
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| NAT1196 | National Diagnostics | 1L | EUR 98.4 |