Biomolecular Binding Kinetics Assay

Cereblon Binding Assay Kit

79899 96 rxns.
EUR 600
Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Binding Assay Laboratories manufactures the biomolecular binding kinetics assay reagents distributed by Genprice. The Biomolecular Binding Kinetics Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact binding assay. Other Biomolecular products are available in stock. Specificity: Biomolecular Category: Binding Group: Kinetics Assay

CytoSelect™ Cell Viability and Cytotoxicity Assay

96 assays
EUR 305

Cell Proliferation and Cytotoxicity MTT Assay Kit

100 Assays
EUR 198

Cell Proliferation and Cytotoxicity MTT Assay Kit

500 Assays
EUR 360

Cell Proliferation and Cytotoxicity MTT Assay Kit

5000 Assays
EUR 1485.6

PDE1A Assay Kit

96 rxns.
EUR 405
Description: The PDE1A Assay Kit is designed for identification of PDE1A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1A1 to the binding agent. The key to the PDE1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1A1 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader._x000D_

PDE1B Assay Kit

96 rxns.
EUR 405
Description: The PDE1B Assay Kit is designed for identification of PDE1B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1B to the binding agent. The key to the PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1B for 1 hour. Second, a binding agent is_x000D_added to the reaction mix to produce a change in fluorescent polarization that can then be_x000D_measured using a fluorescence reader equipped for the measurement of fluorescence_x000D_polarization.

PDE1C Assay Kit

96 rxns.
EUR 405
Description: The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are_x000D_required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a_x000D_sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction_x000D_mix to produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader.

Kinetics Assay information

Cell Meterâ„¢ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*

20111 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*

20111-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence*

20113 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence*

20113-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*

20115 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*

20115-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*

20117 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*

20117-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*

20119 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*

20119-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*

20125 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*

20125-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 3/7 Binding Assay Kit *Red Fluorescence*

20101 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 3/7 Binding Assay Kit *Red Fluorescence*

20101-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*

20100 25 Tests
EUR 341
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*

20100-25Tests 25 Tests
EUR 334
Description: Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

Cell Meterâ„¢ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

22837-100Tests 100 Tests
EUR 222
Description: Annexin V may be conjugated to fluorochromes including APC.