Month: July 2021

July 13, 2021

Plasmids & Vectors

Plasmid vectors and detailed instructions for subtilase expression are available on request from the Wells laboratory. Subtiligase is expressed in Bacillus subtilis and the enzyme is secreted to the media. The enzyme is purified through ammonium sulfate precipitation, anion exchange, isopropyl resin capture (for the catalytic cysteine residue in subtilase), and gel filtration. The enzyme is stored at −80 °C and retains activity for at least 2 years after purification. […]